Green Flueresoent Protein
a.Green Flueresoent Protein EFF fluoreecea green under cerlain light wavelength-ac In the tab,
researchers often "hiae" the gene for 131"? to a gone coding [or a. protein of interest. Then the EdEI'Lfial; can see where in acell their protein residcahaaed cm. the green m1“ Remember, the will onlyfluereacea iEit is ill—111:.P synthesized. a- In the table below. write the lettEI of the location in which EIEETL code-i1-
will be- detected it'LitiaJljl-I dun'ng protein synthesis and other localization
final deafinalien- Each box 1will have m answer and game answers
may.r he used more than once. Protein Attached to Starting location of Final destination of
(3H! :4 een fluorescence ;-e11 fluorescence - a... ........... ——
ii.2] Rhesus Factor .._.................. —
iv- [2‘] I—Iea‘tr.r chain of
hnlihod '._-:-.. |"-' b. 2 Imagine you created a person with the antibody light chain-gene - .
fused to EFF in their genome- Name a mac of cell that would 1;] ow green. _ 'I"——— Location: A. Cytoplaatn B. Rough Eli
C- Smeoth ER
D- Mitochondria. E. Plasma Membrane
-l-":'i'~l11cle1.ia '
G. Dulaide of cell .- -'I _. 1:- ['2]- Irnagine a virus entered the person in EIJ. WEI-old [115* total amount
of green in their Eerie increase or decrease during the immune reeponee?‘ ti. [1 Pete: a macrophage eats a pathogen coated with antibody in the person in at. which of the
following are bonds in SF!" that get htolcen clown? Circle ALL that apply] - hjrclrogen bond: ': - ELIE linkages - pep-tide bunch. - plifisphodieateriloonda e- -1 Imagine a different person who has no immunity and moat live in a sterile germ-free
environment. Chance the mutation below that will cause this severe phonon-tie and egplain how it
does so in 1-2 sentences. i. Mutation in both heavy chain alleles that delete-.5. one of the 51 ".-' segmeflte Welter-tin both heavy chain alleles that etiminates moat of the intracellular domain
5—.—
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